Beijing Tsingke Biotech Co., Ltd.

sgRNA guides Cas9 for precise CRISPR gene editing. Tsingke's sgrna synthesis offers higher efficiency, lower off-target effects, and reduced immunogenicity. HIGHLIGHTS OF SGRNA Low cytotoxicity: Low impurity (residual substance) content; High editing efficiency: Increased experimental success rate; Stable quality: Strict quality control between batches; No DNA interference: No unnecessary gene fragment insertion. SERVICE DETAILS OF SGRNA Service Modifications length(nt) Quantity Turnaround Time* Purification QC Deliverable Cas9 sgRNA-OPC First and last 3 bases with 2'-OMe and PS 97~103 1.5 nmol 5 OPC UV& MS Tube or customized lyophilized RNA COA report (electronic) 3 nmol 5 nmol Cas9 sgRNA-HPLC 1.5 nmol 10~12 3 nmol 5 nmol Cas9 crRNA ~36 5 nmol 5~7 HPLC UV& MS& HPLC Cas9 tracrRNA ~67 3 nmol LbaCasl2a crRNA Bases 1-3 and 29-40 (5'-end) and base 32 (3'-end) with 2'-OMe, first and last 3 bases with PS ~40 5 nmol AsCasl2a crRNA ~40 5 nmol LwCas13a...

AAV, LV, and AdV virus packaging , library construction, and cell line development, providing ready-to-use, reliable tools to accelerate gene research and functional studies. VIRUS PACKAGING OVERVIEW Virus packaging services offer high-quality, ready-to-use viral vectors for gene delivery, functional genomics, gene therapy studies, and CRISPR or shRNA library experiments. Our services cover a comprehensive suite of solutions for genetic research, including Adeno-Associated Virus (AAV) packaging, Lentivirus (LV) packaging, Adenovirus (AdV) packaging, library construction, and cell line construction. Our viral vectors are produced under strict quality control, ensuring high titer, purity, and safety, while offering fast production, reproducibility, scalability, and endotoxin-free preparations. Combined with expert support for custom library generation and reliable cell line construction, we provide end-to-end solutions that streamline your experiments, accelerate research workflows, an...

Precise introduction of point mutations, deletions, or insertions into your DNA sequence with full sequence verification. HIGHLIGHTS OF SITE-DIRECTED MUTAGENESIS self directed mutagenesis enables precise introduction of specific DNA mutations, including point mutations, insertions, or deletions. It is widely applied in protein functional site studies, enzyme activity optimization, DNA element functional analysis, and gene therapy research. Tsingke provide professional and cost-effective site-directed mutagenesis services, accurately introducing desired mutations into any gene template at any number of sites, significantly enhancing experimental efficiency and reducing research costs. SERVICE DETAILS OF SITE-DIRECTED MUTAGENESIS Length Turnaround time (Calendar Day) Vector Deliverables < 1.5 kb 7-10 Customer-specific (1) 1 tube of lyophilized plasmid DNA (about 1~4 μg/tube); (2) QC files: Sequencing map (.abl file); Target sequence (.seq file); COA Report (elec tronic); (3) Any ...

siRNAs are 21-23 nt duplexes that guide RISC to cleave target mRNA. Tsingke provides conventional and modified siRNA from µg to kg scale. HIGHLIGHTS OF sirna duplex MS molecular weight detection with an error margin of less than 0.1% HPLC purity detection ensures purity levels greater than 90%; A high modification rate is achieved, and stable groups such as Ps, 2-F, 2-OMe, and FAM are incorporated into the siRNA oligonucleotides to ensure suitability for downstream applications. Leverage Tsingke's comprehensive industry chain advantages to provide fast and cost-effective services; Multi-channel synthesizer with μg to kg level synthesis capabilities, synthesizing over 100,000 siRNA oligos annually. SERService Details Length(nt) Quantity Purification QC Deliverable siRNA Custom siRNA synthesis 20~25 5 nmol or 2 OD HPLC HPLC Tube or customized lyophilized RNA COA report (electronic) siRNA NC Various types available: negative/positive control siRNA, transfection control siRNA, et...