SGRNA



sgRNA guides Cas9 for precise CRISPR gene editing. Tsingke's sgrna synthesis offers higher efficiency, lower off-target effects, and reduced immunogenicity.



HIGHLIGHTS OF SGRNA

Low cytotoxicity: Low impurity (residual substance) content;



High editing efficiency: Increased experimental success rate;



Stable quality: Strict quality control between batches;



No DNA interference: No unnecessary gene fragment insertion.



SERVICE DETAILS OF SGRNA

Service Modifications length(nt) Quantity Turnaround Time* Purification QC Deliverable

Cas9 sgRNA-OPC First and last 3 bases with 2'-OMe and PS 97~103 1.5 nmol 5 OPC UV& MS Tube or customized lyophilized RNA

COA report (electronic)

3 nmol

5 nmol

Cas9 sgRNA-HPLC 1.5 nmol 10~12

3 nmol

5 nmol

Cas9 crRNA ~36 5 nmol 5~7 HPLC UV& MS& HPLC

Cas9 tracrRNA ~67 3 nmol

LbaCasl2a crRNA Bases 1-3 and 29-40 (5'-end) and base 32 (3'-end) with 2'-OMe, first and last 3 bases with PS ~40 5 nmol

AsCasl2a crRNA ~40 5 nmol

LwCas13a crRNA None ~55 5 nmol

PspCasl3b crRNA ~56 5 nmol

RxCasl3d crRNA ~43 5 nmol

CASES OF SGRNA

The sample of this custom RNA oligos is a 100 nt sgRNA with three 2'-OMe modifications at both ends. Mass spectrometry and chromatography analyses confirm its sequence accuracy and a high purity of 96.54%.



For more information about sgrna plasmid, please feel free to contact us!







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